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Cytiva TempliPhi™ Sequence Resolver Kit
Uses modified nucleotides in isothermal rolling circle amplification (RCA) to improve results with difficult to sequence templates
Brand: Cytiva 28-9035-29
Includes: Sample buffer, Reaction buffer and Enzyme mix
Description
- Cultureless preparation from bacterial colonies, glycerol stock or purified DNA, saves time and reagents
- Requires 20 minutes hands-on time
- Excellent sequence finishing performance resolving sequencing stop and compression issues associated with difficult templates
- Improve difficult template sequencing success with up to 820 bp Phred 20 read length
- For use directly in cycle sequencing reactions without purification
- Available in sizes of 20, 50 and 500 reactions
Specifications
| Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°CThaw components on ice and maintain at 0°C to 4°C during handling | |
| Amplification of difficult templates for successful DNA sequencing | |
| 20 | |
| 18 h |
| illustra TempliPhi Sequence Resolver Kit uses the highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides to amplify circular templates for subsequent sequencing. | |
| 1 x 90μL Sample Buffer, 1 x 90μL Reaction Buffer, 1 x 90μL Enzyme mix | |
| illustra TempliPhi Sequence Resolver Kit |
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