Thermo Scientific™ GeneJET Endo-Free Plasmid Maxiprep Kit

Description

The Thermo Scientific GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 μg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights:

• Efficient—high yields up to 750 μg of high quality plasmid DNA from 250 mL of bacterial culture
• High concentration of DNA purified—> 400 ng/μL
• Convenient—spin column format, centrifuge or vacuum protocols
• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

• Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• Transfection of robust cell lines
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription

Includes

• Resuspension Solution
• Lysis Solution
• Neutralization Solution
• Endotoxin Binding Reagent
• Wash Solution I (concentrated)
• Wash Solution II (concentrated)
• RNase A Solution
• Elution Buffer (10 mM Tris-HCl, pH 8.5)
• GeneJET Maxi Purification Columns pre-assembled with collection tubes (50 mL)
• Collection tubes (50 mL)

Hazardous: No

Quality Control:

• The GeneJET Plasmid Maxiprep Kit is qualified by isolating high copy number plasmid DNA from 250 mL of overnight E. coli culture (OD600 = 2) grown in LB medium following the protocol outlined in the manual.
• The kit passes QC requirements if the purified plasmid DNA has an A_260/280 ratio between 1.8 and 1.9 and a dominant band of supercoiled plasmid DNA is observed after agarose gel electrophoresis.
• The functional quality of the plasmid DNA is evaluated by digestion with restriction endonucleases.

Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:

• Transfection of robust cell lines
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.