Thermo Scientific™ Reagents for GeneJET™ Plasmid Miniprep Kit

Description

Thermo Scientific™ GeneJET™ Neutralization Solution and Lysis Solution are components of the GeneJET Plasmid Miniprep Kit (K0502/K0503) and may be purchased separately.

The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20μg of high copy plasmid DNA per isolation procedure.

The Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 μg of high copy plasmid DNA per isolation procedure.

Highlights

• Efficient - high yields of up to 20 μg of high quality plasmid DNA
• Fast - procedure takes less than 14 minutes
• Convenient - no phenol/chloroform extraction or alcohol precipitation required
• Pure - purified DNA is immediately ready to use

Applications
Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:

• FastDigest or conventional restriction digestion
• Automated fluorescent and radioactive sequencing
• PCR
• In vitro transcription
• Transformation

Principle
A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing.

Components

• Resuspension Solution
• Lysis Solution
• Neutralization Solution
• Wash Solution (concentrated)
• RNase A Solution
• Elution Buffer
• Spin Columns
• Collection Tubes
• Detailed Protocol

Hazardous: No
Quality Control:

• The kit is tested for the isolation of pUC19 DNA from E. coli according to the protocol.
• The quality of the isolated DNA is evaluated by spectrophotometric assay, agarose gel electrophoresis, digestion with FastDigest restriction enzymes, and automated sequencing.

Storage Condition: Ambient,-20°C

Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:—FastDigest or conventional restriction digestion,—automated fluorescent and radioactive sequencing