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Thermo Scientific™ Phire Green Hot Start II PCR Master Mix
Thermo Scientific Phire Green Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps.
Brand: Thermo Scientific™ F126L
6606.48 SEK valid until 2024-08-31
Use promo code "21655" to get your promotional price.
Additional Details : Weight : 0.09000kg
Includes: Phire Hot Start II DNA Polymerase is supplied with 5x Phire Reaction Buffer and DMSO. The buffer provides 1.5 mM MgCl2 in the final 1x concentration.
Description
Thermo Scientific Phire Green Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to PCR reaction. The master mix also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
Phire Hot Start II DNA Polymerase, inluded in the master mix, is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase (Cat. No. EP007).
Highlights
- Quick hot start—No reactivation step
- Fast enzyme—Amplify four times faster than with hot start Taq
- Robust—Minimal reaction optimization due to high inhibitor tolerance
- High yields—Abundant products due to high efficiency
- Longer PCR products—Amplify significantly longer DNA fragments than with any hot start Taq
- Direct loading on gel
Applications
- Hot Start PCR
- Routine PCR
- Non-high fidelity PCR
- Fast PCR
- High throughput PCR
- Genotyping
Note
The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases. For optimal results start by accurately calculating your Tm with our Tm calculator.
Specifications
• 2X Phire Green Hot Start II PCR Master Mix, 20 x 1.25 mL • DMSO, 4 x 0.5 mL • Nuclease-free water, 20 x 1.25 mL Store at -20°C. |
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Phire Hot Start II DNA Polymerase | |
Hot Start PCR Master Mix | |
Cloning, Hot-start PCR | |
2X | |
1000 Reactions | |
SuperMix or Master Mix |
Medium | |
Fast | |
Wet or Dry Ice | |
2X | |
Built-In Hot Start | |
Blunt | |
DNA |
For Research Use Only. Not for use in diagnostic procedures.