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Trypsin, 1-250 from Porcine Pancreas , MP Biomedicals, LLC

Product Code. 15782412
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Quantity:
20 g
Packaging:
Glass
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Trypsin, 1-250 from Porcine Pancreas has an activity >250 USP u/mg. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues.

  • The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture
  • The 1:250 designation indicates the tryptic activity based on standardized testing
  • This ratio indicates that one part trypsin will digest 250 parts casein in the USP activity assay
  • Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain
  • Trypsin is used to re-suspend cells adherent to the cell culture dish wall during the process of harvesting cells
  • Trypsin can also be used to dissociate dissected cells
  • Trypsin can be used to break down casein in breast milk
  • Trypsin is commonly used in biological research during proteomics experiments to digest proteins into peptides for mass spectrometry analysis
  • Trypsin can also be used to dissolve blood clots in its microbial form and treat inflammation in its pancreatic form
  • One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified
TRUSTED_SUSTAINABILITY

Specifications

CAS 9002-07-7
Color White
pH ∼8.0
Packaging Glass
Molecular Formula C35H47N7O10
Quantity 20 g
Synonym tryptase, trypsin, tryptase from human lung, tryptase from human lung, buffered aqueous solution, ≥5 units/mg protein, acetic acid; tert-butyl n-2s-1-2s-1-2s-5-diaminomethylideneamino-1-5-methyl-2-oxochromen-7-yl amino-1-oxopentan-2-yl amino-3-hydroxy-1-oxopropan-2-yl amino-1-oxo-3-phenylpropan-2-yl carbamate
InChI Key WGWZNYKOUXOZTC-UHFFFAOYNA-N
Molecular Weight (g/mol) 725.80
PubChem CID 5311489

Safety and Handling

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Product Identifier
  • Trypsin, 1-250
 Signal Word
  • Danger
 Hazard Category
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2
  • Serious eye damage/eye irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P284-¢In case of inadequate ventilation! wear respiratory protection.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P405-Store locked up.
  • P501-Dispose of contents/container to in accordance with local/regional/national/international regulations..
  • P321-Specific treatment (see on this label).
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : trypsin
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