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Invitrogen™ Vivid™ CYP3A4 Red Screening Kit

Vivid™ CYP3A4 Red Screening Kit

Brand: Invitrogen™ P2856

Product Code. 10184852

4660.00 SEK / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Vivid™ CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. They provide an optimized method for studying isozyme-specific CYP450-drug interactions, metabolism, and inhibition. Vivid™ CYP450 Screening Kits offer:

Easy three-step procedure, 'mix and read' format, reactions at room temperature or 37°C

Superior fluorescent properties and kinetics compared to conventional fluorogenic probes

High signal-to-background ratio, broad dynamic range

Compatible with multiple assay formats from 96-well to 1536-well

Simple Mix and Read Kit Format
Vivid™ CYP450 Screening Kits include Vivid™ Substrate, Vivid™ Fluorescent Standard, reaction buffer, Vivid™ Regeneration System, NADP+, and CYP450 BACULOSOMES™ Plus Reagents. The CYP450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme (CYP3A4 in this case) and human cytochrome-P450 reductase. CYP450 BACULOSOMES™ Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

Unique Vivid™ Reagents for Bright Fluorescent Signals and Low Background
Vivid™ Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product (Figure 1). They have superior fluorescence, solubility, and kinetic properties compared to conventional fluorogenic probes. This results in higher sensitivity, greater signal-to-noise ratio, and better assay reproducibility. Vivid™ BOMR Substrate is the substrate included in this particular kit. This substrate yields a product that emits a red fluorescence.

Flexible Assay Formats for Optimized Results
The sensitivity of the Vivid™ CYP450 assays allows detection of weak inhibitors and miniaturization to as little as 2μl per reaction. Assays may be set up in kinetic mode or in end-point mode to facilitate multi-plate screening (Figure 2). Assays may be performed at room temperature or 37°C.

Applications: high-throughput screening of enzyme-drug interactions, compound profiling for drug inhibition of cytochrome P450 isozymes, generation of predictive SAR models to guide compound acquisition

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

Specifications

For Use With (Equipment)	Microplate Reader
Content And Storage	Vivid™ CYP450 Screening kits include Vivid™ CYP450 Fluorogenic substrates, BACULOSOMES™ CYP450 reagents, fluorescent standards, and an NADPH-regeneration system. Vivid™ CYP450 Fluorogenic substrates are also available separately. Store reaction buffers at +4°C or room temperature. Store BACULOSOMES™, the NADPH-regeneration system, and NADP+ at -80°C. Store the Vivid™ substrates and standards at +4°C, protected from light. (For long-term storage, resuspend in organic solvent and keep at -20°C.)
Label or Dye	VIVID Red
Quantity	>300 assay(s)
Color	Red

Product Line	VIVID™
Detection Method	Fluorescence
Product Type	Screening Kit
Format	96-well Plate
Shipping Condition	Dry Ice

Product Suggestions Documents

Documents

Scientific Resources

Brochures

2005 JBS miniaturized P450 assays File size: 462.6 kB

Product Information

Manuals

Vivid CYP450 Screening Kits Version: 24 April 2012 File size: 738.6 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number3070394	Certificate TypeCertificate of Analysis	Date21/02/2025	Catalog NumberP2856
Lot Number2945677	Certificate TypeCertificate of Analysis	Date05/04/2024	Catalog NumberP2856
Lot Number2808800	Certificate TypeCertificate of Analysis	Date26/09/2023	Catalog NumberP2856
Lot Number2636072	Certificate TypeCertificate of Analysis	Date07/02/2023	Catalog NumberP2856
Lot Number2550290	Certificate TypeCertificate of Analysis	Date17/01/2023	Catalog NumberP2856

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