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Invitrogen™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide
Z'-LYTE™ Kinase Assay Kit - Ser/Thr 1 Peptide
Brand: Invitrogen™ PV3174
This item is not returnable.
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Description
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a porcine pyruvate liver kinase derived peptide used to study PKA substrate specificity, and containing an R-R-X-S consensus sequence, where X is any residue.Specifications
Z´-LYTE™ | |
FRET | |
Z´-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions. | |
Methylcoumarin | |
800 x 20 μL Assays | |
porcine pyruvate liver kinase (LRRASLG) | |
800 Reactions | |
End Point | |
Peptide-Based Substrate | |
AURKA, AURKB, PRKACA, PRKG1, PRKG2, PRKX, RPS6KA4, RPS6KA5 |
Serine/Threonine Kinases | |
Serine/Threonine Kinase Assay | |
Biochemical phospho-specific activity (no antibody) | |
Kinase Assay Kit | |
384-well plate | |
405⁄465, 535 | |
BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978) | |
Dry Ice | |
PRKX, PRKG2, RPS6KA5, PRKACA, PRKG1, RPS6KA4, AURKB, AURKA |
For Research Use Only. Not for use in diagnostic procedures.