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Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

Brand: Thermo Scientific™ PV3176

Product Code. 12020176

7500.00 SEK / 800 reactions

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For Research Use Only. All usage must comply with product instructions.

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Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon bovine MBP Thr-97 derived peptide, containing a P-X-(S/T)-P consensus sequence, where X is any residue.

Specifications

Description	Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide, 800 x 20μL Assays, 800 rxns, 405/465nm Excitation, 535nm Emission, Methylcoumarin Label, FRET Technique, Serine/Threonine Kinases Target Enzyme, Peptide-Based Substrate Properties, End Point Readout, Used For Pharma and Biopharma, Protein Assays and Analysis
For Use With (Application)	Pharma and biopharma, protein assays and analysis, target and lead identification and validation, FRET
Sample Volume	800 x 20μL
Assay Entry	Biochemical phospho-specific activity (no antibody)
Product Type	Z'-LYTE™ Kinase Assay Kit - Ser/Thr 3 Peptide
Format	384-well plate
Label or Dye	Methylcoumarin
Readout	End Point
Shipping Condition	Dry Ice
Substrate Properties	Peptide-Based Substrate
Target Enzyme	Serine/Threonine Kinases

Product Line	Z ́-LYTE™
Includes	All necessary reagents for performing primary or secondary screens
Assay	Serine/Threonine Kinase Assay
Content And Storage	–80°C, Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
Quantity	800 x 20μL, 800 x 20 μL Assays
Excitation/Emission	405/465, 535 nm
No. of Reactions	800 x 20 μL Reactions
References	BBRC vol. 61, pp 559-563 (1974), JBC, vol 252, p 4888 (1978)
Source	Bovine MBP, Thr-97 Derived Peptide S5, Consensus Seq Pro-X-(Ser⁄Thr)-Pro (APRTPGGRR)
Target Entry	MAPK13, MAP2K1, MAP2K6, MAPK1, MAP3K8, BRAF, MAP2K2, MAPK12, MAPK3, BRAF V599E
Validated Kinases	BRAF, MAPK1, MAPK3, MAPK12, MAPK13, MAP2K1, MAP2K2, MAP3K6, MAP2K8

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Documents

Scientific Resources

Brochures

Z’-LYTE™ fluorescent kinase assay technology File size: 692.4 kB

Expanding Z´-LYTE™ File size: 155.6 kB

Product Information

Manuals

Z-LYTE Kinase Assay Kit Ser/Thr 3 Peptide Protocol Version: Rev B 04/05 File size: 334.1 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2981658	Certificate TypeCertificate of Analysis	Date21/02/2025	Catalog NumberPV3176
Lot Number2917615	Certificate TypeCertificate of Analysis	Date07/03/2024	Catalog NumberPV3176
Lot Number2917615	Certificate TypeCertificate of Analysis	Date07/03/2024	Catalog NumberPV3176
Lot Number2744075	Certificate TypeCertificate of Analysis	Date30/06/2023	Catalog NumberPV3176
Lot Number2672159	Certificate TypeCertificate of Analysis	Date21/03/2023	Catalog NumberPV3176

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