Test
Total Exosome Isolation Reagent (from cell culture media) enables fast and efficient enrichment of intact exosomes from cell culture media samples.
Gibco™ Exosome-Depleted FBS streamLines and standardizes cell culture workflows for exosome research. It is ready to use and performance tested to help save time and enable optimal exosome preparations.
Shipping Condition | Frozen |
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Content And Storage | Storage conditions: ≤-10°C Shipping conditions: Frozen |
Product Type | Fetal Bovine Serum |
Form | Liquid |
Serum Treatment | Exosome-depleted |
Sterility | Sterile |
Country of Origin | United States |
Age | Fetal |
Exosome-Human CD63 Isolation/Detection Reagent enables exosome (also called extracellular vesicles and multi-vesicular bodies) detection using techniques such as flow cytometry, electron microscopy, or Western blotting.
The Total Exosome RNA and Protein Isolation Kit enables isolation of total RNA and proteins from exosome samples extracted from biological fluids (e.g., by using a Total Exosome Isolation (from cell culture media) or Total Exosome Isolation (from serum).
Fast and efficient enrichment of exosomes from blood serum and plasma samples for the most efficient total RNA isolation or other biological studies. Includes RNase-free water.
Particle Size | 350 to 150 nm |
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Includes | Exome Precipitation Solution; RNase-free Water |
Composition | Polyethylene glycol with chemicals; Water |
Content And Storage | Room Temperature (18°C to 25°C) |
Donor Source | Serum or Plasma Samples |
Form | Liquid |
Color | Colorless |
Sufficient For | 0.1 to 1 mL |
Shelf Life | At least 1 year |
For Use With (Application) | Enrichment of exosomes from blood serum and plasma samples |
Odor | Odorless |
Highly pure, lyophilized exosome standards with superior stability, optimal for multiple applications
Content And Storage | Store at 4°C. After reconstitution store at -70°C. |
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Concentration | LYOPH |
Total Exosome Isolation Reagent (from serum) enables fast and efficient enrichment of intact exosomes from blood serum samples with our simple and reliable protocol.
The Total Exosome Isolation Kit (from plasma) consists of two parts: Total Exosome Isolation Reagent (from plasma) and Proteinase K (20 mg/mL).
Rabbit Polyclonal Antibody has been used in 3 publications
Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
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Target Species | Human,Mouse,Chicken |
Host Species | Rabbit |
Conjugate | Unconjugated |
Applications | Western Blot,ChIP Assay,Immunohistochemistry,Immunocytochemistry,Immunofluorescence |
Isotype | IgG |
Gene Accession No. | Q06265, Q9JHI7 |
Concentration | 0.6 mg/mL |
Antigen | Exosome Component 9 |
Gene Symbols | EXOSC9 |
Regulatory Status | RUO |
Purification Method | Affinity Purified |
Dilution | Western Blot 1:5000, Simple Western 1:1000, Chromatin Immunoprecipitation reported in scientific literature (PMID 27543448), Immunohistochemistry 1:50-1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation reported by customer review, Immunohistochemistry-Paraffin 1:50-1:100, Chromatin Immunoprecipitation (ChIP) |
Gene Alias | Autoantigen PM/Scl 1, EC 3.1.13, exosome component 9Polymyositis/scleroderma autoantigen 75 kDa, p6, PM/Scl-75P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, PMSCL175kD, Polymyositis/scleroderma autoantigen 1, polymyositis/scleroderma autoantigen 1 (75kD), polymyositis/scleroderma autoantigen 1, 75kDa |
Gene ID (Entrez) | 5393 |
Formulation | PBS and 30% Glycerol with 0.05% Sodium Azide |
Immunogen | A genomic peptide made to an internal region of human Exosome Component 9 (within residues 250-439). [Swiss-Prot Q06265] |
Classification | Polyclonal |
Primary or Secondary | Primary |
Test Specificity | Human, Mouse and Chicken. Immunogen has 83% identity to rat and 90% identity to bovine. |
Highly pure, lyophilized exosome standards with superior stability, optimal for multiple applications
Content And Storage | Store at 4°C. After reconstitution store at -70°C. |
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Concentration | LYOPH |
pASK-IBA E. coli expression vectors encoding a Strep-tag™II and chloramphenicol resistance
Promoter | Tet |
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Antibiotic Resistance Bacterial | Chloramphenicol |
Format | Suspension |
Replication Origin | F1 and ColE1 origin |
Storage Buffer | TE buffer |
Cloning Method | Direct Cloning |
Concentration | 250 ng/μL |
Size Range | approx. 3 kb |
Expression System | E. coli |
Protein Tag | Strep-tag II |
Exosome Immunoprecipitation Reagent (Protein G) enables fast and gentle magnetic isolation of exosomal proteins, causing minimal physical stress to the target protein and allowing comparison of multiple samples on the same gel.
Exosome Immunoprecipitation Reagent (Protein A) enables gentle magnetic isolation of exosomal proteins, causing minimal physical stress to the target protein.
Exosome-Streptavidin Isolation/Detection Reagent, when combined with your choice of biotinylated primary antibody, enables the purification of exosomes that can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting.
Total Exosome Isolation Reagent from urine enables fast and efficient enrichment of intact exosomes with a protocol that is scalable depending on sample size.