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ELISA Substrates and Reagents

ELISA Substrates and Reagents

Colorimetric, chemiluminescent and chemifluorescent substrates for alkaline phosphatase (AP), horseradish peroxidase (HRP) and other enzyme reporter systems; includes several sensitive formulations of TMB for kinetic, competition or sandwich ELISA.
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1 – 15 of 1,006 results
1

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

EDGE
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
2

Invitrogen™ ELISA Stop Solution

A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.

EDGE
Shipping Condition Wet Ice
Content And Storage Store at 2°C to 8°C
Form Liquid
Product Type ELISA Stop Solution
Substrate Type HRP, TMB
3

Thermo Scientific™ ELISA Carbonate Coating Buffer

For use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA

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4

Invitrogen™ eBioscience™ ELISA/ELISPOT Diluent (5X)

Supplemented with fetal bovine serum as source of animal serum proteins. Used for blocking non-specific binding of ELISA plates, diluting recombinant standards and test samples and making the working dilutions of secondary detection reagent. Also contains 1% ProClin 150 as a preservative

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5

Thermo Scientific™ Stop Solution for TMB Substrates

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

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6

Thermo Scientific™ SuperSignal™ ELISA Femto Substrate

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

EDGE
Content And Storage Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature.
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal™
Detection Method Chemiluminescence
8

Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

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9

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

Content And Storage Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal™
Detection Method Chemiluminescence
10

Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

11

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

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12

Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Detection Method Colorimetric
13

Invitrogen™ TMB ELISA Buffer Kit, PeproTech®

This kit contains the key components required for the analysis of 10 Sandwich ELISA plates. It has been specifically formulated to be used in conjunction with PeproTech’s Standard TMB ELISA Development Kits.

14

Thermo Scientific™ QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit

Enable sensitivity comparable to enhanced chemiluminescence (ECL) using the most sensitive fluorescent ELISA substrate for peroxidase (HRP).

15

Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)

Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.