High Fidelity PCR Reagents and Kits
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High Fidelity PCR Reagents and Kits
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Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Content And Storage | • Phusion DNA Polymerase (2 U/μL) • 5X Phusion HF & GC Buffers • DMSO • 50 mM MgCl2 solution Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration. Store at -20°C. |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | Standalone |
Product Type | High-Fidelity DNA Polymerase |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
Concentration | 2 U/μL |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF Buffer
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion High-Fidelity DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | High-Fidelity PCR Master Mix |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Invitrogen™ Platinum™ SuperFi II Green PCR Master Mix
Invitrogen Platinum SuperFi II Green PCR Master Mix (2X) contains a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup, as well as two tracking dyes for direct loading of PCR products on gels.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Dry Ice |
---|---|
Polymerase | Platinum SuperFi II DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | >300X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Platinum™ SuperFi II DNA Polymerase
Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.
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Shipping Condition | Dry Ice |
---|---|
Polymerase | Platinum SuperFi II DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | >300X |
Size (Final Product) | 20 kb or less |
Reaction Format | Standalone |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion™ Hot Start II DNA Polymerase (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion Hot Start II DNA Polymerase |
GC-Rich PCR Performance | High |
Content And Storage | • Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/μL) • 5X Phusion HF & GC Buffers • DMSO • 50 mM MgCl2 solution Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration. Store at -20°C. |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | Standalone |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2 U/μL |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Platinum™ SuperFi II PCR Master Mix
Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.
Shipping Condition | Dry Ice |
---|---|
Polymerase | Platinum SuperFi II DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | >300X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion™ Plus DNA Polymerase
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion Plus DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | >100X |
Size (Final Product) | 20 kb or less |
Reaction Format | Separate components |
Reaction Speed | 15 to 30 sec./kb |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion Flash II DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 25X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | High-Fidelity PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion Hot Start II High-Fidelity PCR Master Mix
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion Hot Start II High-Fidelity DNA Polymerase |
GC-Rich PCR Performance | Medium |
Content And Storage | • 2X Phusion Hot Start II High-Fidelity PCR Master Mix • Nuclease-free water • DMSO 2X Phusion Hot Start II High-Fidelity PCR Master Mix provides 1.5 mM MgCl2 in the final 1X concentration. Store at –20°C. |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | Hot Start High-Fidelity PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Platinum™ Taq DNA Polymerase, high fidelity
Platinum™ Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Dry Ice |
---|---|
Polymerase | Platinum Taq DNA Polymerase High Fidelity |
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 6X |
Size (Final Product) | 20 kb or less |
Reaction Format | Separate Components |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Detection Method | Primer-probe |
Thermo Scientific™ Phusion High-Fidelity PCR Kit
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion High-Fidelity DNA Polymerase |
GC-Rich PCR Performance | High |
Content And Storage | • Phusion DNA Polymerase (2 U/μL) • 5X Phusion GC & HF Buffer • dNTP mix (10 mM each) • Control template (λ DNA) and DNA size standard • 1.3 kb & 10 kb primers (4 μM each) • 50 mM MgCl2 solution and DMSO Store at –20°C. |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | Separate Components |
Product Type | High-Fidelity PCR Kit |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ Phusion™ Plus PCR Master Mix
Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion Plus DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | >100X |
Size (Final Product) | 20 kb or less |
Reaction Format | Ready to use master mix |
Reaction Speed | 15 to 30 sec./kb |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ AccuPrime™ Taq DNA Polymerase, high fidelity
Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates.
Shipping Condition | Dry Ice |
---|---|
Polymerase | AccuPrime Taq DNA Polymerase High Fidelity |
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 9X |
Size (Final Product) | 20 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Detection Method | Primer-probe |
Thermo Scientific™ Phusion Green High-Fidelity DNA Polymerase (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion High-Fidelity DNA Polymerase |
GC-Rich PCR Performance | High |
Content And Storage | • Phusion DNA Polymerase, 2 U/μL • 5X Phusion Green HF and GF Buffers • DMSO • 50 mM MgCl2 Phusion Green HF and Phusion Green GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration. Store at -20°C. |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | Standalone |
Product Type | High-Fidelity DNA Polymerase |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
Concentration | 2 U/μL |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with GC Buffer
Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.
Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion High-Fidelity DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | High-Fidelity PCR Master Mix |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Standard PCR,High-fidelity PCR |