Hot Start PCR Reagents and Kits
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Hot Start PCR Reagents and Kits
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Invitrogen™ Platinum™ Taq DNA Polymerase
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
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Polymerase | Platinum Taq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Format | Tube |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Starting Material | DNA |
For Use With (Application) | Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
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AmpliTaq Gold™ 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.
Shipping Condition | Room Temp or Wet Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Green Features | Sustainable packaging |
Concentration | 2X |
For Use With (Application) | Cloning,Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | AmpliTaq Gold 360 DNA Polymerase |
Format | Tube |
Size (Final Product) | 5 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | PCR Master Mix |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Starting Material | DNA |
Invitrogen™ Platinum™ II Hot-Start PCR Master Mix (2X)
Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.
Thermo Scientific™ Phire Hot Start II DNA Polymerase
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications.
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Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2X |
Purity or Quality Grade | DHPLC |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Cloning,Hot-start PCR |
Thermo Scientific™ Phire Green Hot Start II DNA Polymerase
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.
Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2X |
Reaction Format | Separate Components |
Product Type | Hot Start High-Fidelity DNA Polymerase |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Cloning,Hot-start PCR |
Thermo Scientific™ Phire Green Hot Start II PCR Master Mix
Thermo Scientific Phire Green Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps.
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Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2X |
Reaction Format | SuperMix or Master Mix |
Product Type | Hot Start PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Cloning,Hot-start PCR |
Applied Biosystems™ Platinum™ Multiplex PCR Master Mix
The Platinum™ Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR.
Shipping Condition | Dry Ice |
---|---|
Polymerase | Platinum Taq DNA Polymerase |
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Size (Final Product) | 2.5 kb or less |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,Multiplex PCR |
Multiplex Capability | Up to 20-plex |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2
AmpliTaq Gold™ DNA Polymerase is a chemically modified form of AmpliTaq™ DNA Polymerase requiring thermal activation.
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Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
Fidelity (vs. Taq) | 1X |
Size (Final Product) | 5 kb or less |
Product Type | DNA Polymerase |
Overhang | 3'-A |
Concentration | 5 U/μL |
Exonuclease Activity | 5' - 3' |
Thermo Scientific™ Phire Hot Start II PCR Master Mix
Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2X |
Reaction Format | SuperMix or Master Mix |
Product Type | Hot Start PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Cloning,Hot-start PCR |
Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase
Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.
Polymerase | DreamTaq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Reaction Format | Separate Components |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Hot-start PCR |
Invitrogen™ Platinum™ Taq DNA Polymerase, DNA-free
Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Hot-start PCR |
Polymerase | Platinum Taq DNA Polymerase |
Format | Tube |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | Taq DNA Polymerase |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Starting Material | DNA |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2
AmpliTaq Gold™ DNA Polymerase is a chemically modified form of AmpliTaq™ DNA Polymerase requiring thermal activation.
Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Concentration | 5 U/μL |
Exonuclease Activity | 5' - 3' |
Invitrogen™ Platinum™ II Hot-Start PCR Master Mix (2X)
Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.
Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
The Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.
Shipping Condition | Room Temp or Wet Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Green Features | Sustainable packaging |
Concentration | 5 U/μL |
For Use With (Application) | Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | AmpliTaq Gold 360 DNA Polymerase |
Format | Tube |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Starting Material | DNA |
Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase
AccuPrime™ GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.
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Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | AccuPrime GC-Rich DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2X |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
For Use With (Application) | Hot-start PCR |