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Thermo Scientific™ NHS-Fluorescein (5/6-carboxyfluorescein succinimidyl ester), mixed isomer
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
Content And Storage | Store desiccated at -20°C, protect from moisture |
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Description | Pierce NHS-Fluorescein |
Product Type | NHS-Fluorescein |
Label or Dye | FITC (Fluorescein) |
Reactive Moiety | Active Ester, Succinimidyl Ester, NHS Ester |
Solubility | DMF (Dimethylformamide) |
Emission | 518 |
Excitation | 494 |
Label Type | Classic Dyes |
Chemical Reactivity | Amine |
Product Line | Pierce™ |
Thermo Scientific™ NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester), mixed isomer
Label antibodies, proteins and other molecules for fluorescence imaging (ex 546; em 572-615) using these activated derivatives of red-fluorescent rhodamine dye.
Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Gibco™ Collagenase, Type IV, powder
Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen
Gibco™ Collagenase, Type II, powder
Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen
Thermo Scientific™ Pierce™ TCEP-HCl, No-Weigh™ Format
Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).
Form | Solid |
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Thermo Scientific™ 8M Guanidine-HCl Solution
Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.
Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Shipping Condition | Wet Ice |
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Content And Storage | Upon receipt store desiccated at -20°C. |
Description | EDC |
Cleavable | No |
Water Soluble | Yes |
Form | Powder |
Molecular Weight (g/mol) | 191.7 |
Solubility | Water |
Labeling Method | Chemical Labeling |
Crosslinker Type | Heterobifunctional |
Chemical Reactivity | Amine-Carboxyl |
Cell Permeability | No |
Format | Standard, Single-use, Premium-grade |
Product Type | Crosslinker |
Spacer | Short (<10 Å) |
Reactive Moiety | Carbodiimide |
Spacer Arm Length | 0.0 Å |
PEGylated | No |
Invitrogen™ CellTrace™ Violet Cell Proliferation Kit, for flow cytometry
CellTrace™ Violet Cell Proliferation Kit, for flow cytometry
Shipping Condition | Room Temperature |
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Format | Tube(s) |
Product Type | Cell Proliferation Kit |
For Use With (Equipment) | Flow Cytometer |
Solubility | DMSO (Dimethylsulfoxide) |
Emission | 405⁄450 |
For Use With (Application) | Proliferation Assay |
Product Line | CellTrace™ |
Detection Method | Fluorescence |
Gibco™ Collagenase, Type I, powder
Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Gly-Pro, which is found with high frequency in collagen
Shipping Condition | Room Temperature |
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Content And Storage | Storage conditions: 2°C to 8°C. Protect from light. Shipping conditions: Room temperature Shelf life: 24 months from date of manufacture |
Product Type | Collagenase |
Form | Lyophilized |
Shelf Life | 24 Months |
Sterility | Non-sterile |
Molecular Probes™ Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 488 is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line.
Shipping Condition | Room Temperature |
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Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
Product Type | Dye |
Label or Dye | Alexa Fluor™ 488 |
Emission | 517 nm |
Excitation | 494 nm |
Label Type | Alexa Fluor |
Chemical Reactivity | Amine |
Product Line | Alexa Fluor™ |
Molecular Probes™ Alexa Fluor™ Antibody Labeling Kits
Multiplex with brighter, photostable, Alexa Fluor Antibody Labeling Kits without the intramolecular quenching. Conjugate antibodies to 9 available fluorophores for flow cytometry, ICC, IHC, IF, ELISA or HCS. Each kit contains enough dye to conjugate 100 μg per reaction with 5 reactions per kit.
Shipping Condition | Room Temperature |
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Product Type | Labeling Kit |
Solubility | DMSO (Dimethylsulfoxide) |
Labeling Method | Conjugation-based |
Label Type | Alexa Fluor |
Labeling Scale | 100 μg |
Chemical Reactivity | Amine |
Product Line | Alexa Fluor™ |
Detection Method | Fluorescence |
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit
Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.
Thermo Scientific™ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format
Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.
Molecular Weight (g/mol) | 217.13 |
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Solubility | Water |
PEGylated | No |
Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
Shipping Condition | Ambient |
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Content And Storage | Upon receipt store desiccated at -20°C. |
Description | Sulfo-SMCC |
Cleavable | No |
Water Soluble | Yes |
Molecular Weight (g/mol) | 436.37 |
Solubility | Water |
Labeling Method | Chemical Labeling |
Crosslinker Type | Heterobifunctional |
Chemical Reactivity | Amine-Sulfhydryl |
Cell Permeability | No |
Format | Standard, Single-use, Premium-grade |
Product Type | Crosslinker |
Spacer | Short (<10 Å) |
Reactive Moiety | Maleimide, Sulfo-NHS Ester |
Spacer Arm Length | 8.3 Å |
PEGylated | No |