
Thermo Scientific™ FastDigest EcoRI
The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | EcoR I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest XbaI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Xba I |
Methylation Sensitivity | Dam Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | 5' protruding ends |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ BpiI (BbsI) (10 U/μL)
Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I).

Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | BpiI (BbsI) |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Scarless Cloning |
Compatible Buffer | 10X Buffer G |
Type IIS RE | Yes |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ NTP Set, 100 mM Solution
The Thermo Scientific NTP set is a convenient set of 100 mM solutions of each ATP, CTP, GTP and UTP.

Thermo Scientific™ NotI (10 U/μL)
The NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI).

Concentration | 10 U/μL |
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Thermo Scientific™ pENTR™/D-TOPO™ Cloning Kit
Utilize a highly efficient, 5-minute cloning strategy (TOPO™ Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the Gateway™ System or the MultiSite Gateway™ System

Thermo Scientific™ BsuRI (HaeIII) (10 U/μL)
Cut at GG^CC sites with BsuRI (HaeIII) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: BshFI, BsnI, BspANI, HaeIII, PhoI).

Thermo Scientific™ BshTI (AgeI) (10 U/μL)
Cut at A^CCGGT sites with BshTI (AgeI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AgeI, AsiGI, CspAI, PinAI).

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | BshTI (AgeI) |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer O |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ XbaI (10 U/μL)
Cut at T^CTAGA sites with XbaI restriction enzyme, which functions best at 37°C in Tango buffer.

Concentration | 10 U/μL |
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Thermo Scientific™ FastDigest PaeI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Thermo Scientific™ FastDigest SmaI
The FastDigest SmaI restriction enzyme recognizes CCC^GGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (isoschizomers: TspMI, XmaCI; neoschizomer: XmaI).

Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Sma I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Overhang | Blunt |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ DraI (10 U/μL)
Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.

Thermo Scientific™ PstI (10 U/μL)
The PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI).

Concentration | 10 U/μL |
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Thermo Scientific™ SalI (10 U/μL)
The SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer.

Concentration | 10 U/μL |
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Thermo Scientific™ FastDigest SgsI
Cut at GG^CGCGCC sites with Thermo Scientific™ FastDigest™ AscI (SgsI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: PalAI).
