Thermo Scientific™ DH5α Competent Cells; High Efficiency
High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors
Invitrogen™ SuperScript™ IV Reverse Transcriptase
SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.
Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli
One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.
Invitrogen™ dNTP Set (100 mM)
Invitrogen 100 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water. Each nucleotide is at a concentration of 100 mM.
Thermo Scientific™ FastDigest EcoRI
The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
Invitrogen™ Lipofectamine™ RNAiMAX Transfection Reagent
Provides highest transfection efficiencies on widest variety of cell types for siRNA-mediated gene knockdown experiments
Thermo Scientific™ T4 DNA Ligase (5 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Invitrogen™ UltraPure™ Salmon Sperm DNA Solution
Used directly in preparation of prehybridization and hybridization solutions
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
Invitrogen™ Gateway™ BP Clonase™ II Enzyme mix
Catalyzes in vitro recombination of PCR products to generate entry clones
Thermo Scientific™ FastDigest XbaI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ Tubes, 0.2 mL, flat cap, assorted colors
0.2 mL individual tubes with attached flat or dome snap shut caps, available in a variety of colors
Applied Biosystems™ BigDye XTerminator™ Purification Kit
The BigDye XTerminator™ Purification Kit is a fast, simple purification method for DNA sequencing reactions that removes unincorporated BigDye™ terminators and salts.