beta-Defensin 1 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCCK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BD-1. Anti-Human BD-1-specific antibody was purified by affinity chromatography employing an immobilized Human BD-1 matrix. Sandwich ELISA: To detect Human BD-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BD-1 (500-P253Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BD-1. Western Blot: To detect Human BD-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BD-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis.