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CXCL1 Polyclonal Antibody, PeproTech®, Invitrogen™
Rabbit Polyclonal Antibody
Brand: Invitrogen 500-P92-100UG
This item is not returnable.
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Description
AA Sequence of recombinant protein: ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-alpha/MGSA (CXCL1). Anti-Human GRO-alpha/MGSA (CXCL1)-specific antibody was purified by affinity chromatography employing an immobilized Human GRO-alpha/MGSA (CXCL1) matrix. Sandwich ELISA: To detect Human GRO-alpha/MGSA (CXCL1) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human GRO-alpha/MGSA (CXCL1) (500-P92Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GRO-alpha/MGSA (CXCL1). Western Blot: To detect Human GRO-alpha/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human GRO-alpha/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
Specifications
| CXCL1 | |
| Polyclonal | |
| Unconjugated | |
| Cxcl1 | |
| alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); Cinc1; CINC-1; CXC; CXC motif chemokine; C-X-C motif chemokine 1; C-X-C motif chemokine ligand 1; CXCL; CXCL1; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; fibroblast secretory protein; Fsp; Gro; GRO a; GRO a; Gro1; GRO1 oncogene; GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROa; GROalpha; GRO-alpha(1-73); GRO-alpha(4-73); GRO-alpha(5-73); GRO-alpha(6-73); Growth-regulated alpha protein; GROa; Hematopoietic synergistic factor; HSF; KC; KC(5-72); KC/GR)-alpha; KC/GRO-alpha; KC-T; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; MGSA-a; N51; NAP3; NAP-3; Neutrophil-activating protein 3; platelet-derived growth factor-inducible protein KC; SCYB1; secretory protein N51 | |
| Rabbit | |
| Antigen affinity chromatography | |
| RUO | |
| 2919 | |
| -20°C |
| ELISA, Western Blot | |
| 0.1-1.0 mg/mL | |
| PBS with no preservative | |
| P09341 | |
| Cxcl1 | |
| E.coli-derived Recombinant Human GRO-alpha/MGSA (CXCL1) | |
| 100 μg | |
| Primary | |
| Human | |
| Lyophilized |
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