CXCL1 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-alpha/MGSA (CXCL1). Anti-Human GRO-alpha/MGSA (CXCL1)-specific antibody was purified by affinity chromatography employing an immobilized Human GRO-alpha/MGSA (CXCL1) matrix. Sandwich ELISA: To detect Human GRO-alpha/MGSA (CXCL1) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human GRO-alpha/MGSA (CXCL1) (500-P92Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GRO-alpha/MGSA (CXCL1). Western Blot: To detect Human GRO-alpha/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human GRO-alpha/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.