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Invitrogen™ Gateway™ pENTR™ 1A Dual Selection Vector

Gateway™ pENTR™ 1A Dual Selection Vector

Brand:  Invitrogen™ A10462

Product Code. 10618113

  • 534.00 SEK / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors!

The Gateway™ entry vectors (Table 1) offer the following:
  • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway™ destination vector to ensure cloning of the gene of interest in the correct orientation for expression
  • Kozak consensus sequence for efficient translation initiation in eukaryotic systems
  • Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only)
  • rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli
  • pUC origin for high-copy replication and maintenance of the plasmid in E. coli
  • Kanamycin resistance gene for selection in E. coli
  • The ccdB⁄chloramphenicol fusion gene located between the two attL sites for
    o negative selection and
    o Chloramphenicol selection in E. coli
  • Kanamycin resistance gene for selection in E. coli

    TRUSTED_SUSTAINABILITY
    • Specifications

    Specifications

    Dual SelectionExpression Vector
    Chloramphenicol (CmR), Kanamycin (KanR)
    Untagged
    10 μg
    pENTR™
    10 μg pENTR™ Dual Selection vector, lyophilized in TE, pH: 8.0. Resuspend in water
    Store at -20°C
    No Cleavage Site
    Gateway
    pENTR
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    For Research Use Only. Not for use in diagnostic procedures.