missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. DNA Vectors
  5. 10722144

Invitrogen™ Gateway™ pENTR™ 2B Dual Selection Vector

Gateway™ pENTR™ 2B Dual Selection Vector

Brand:  Invitrogen™ A10463

323.68 SEK valid until 2024-11-30
Use promo code "21651" to get your promotional price.

Product Code. 10722144

  • 578.00 SEK / Each

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers

For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors!

The Gateway™ entry vectors (Table 1) offer the following:
  • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway™ destination vector to ensure cloning of the gene of interest in the correct orientation for expression
  • Kozak consensus sequence for efficient translation initiation in eukaryotic systems
  • Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only)
  • rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli
  • pUC origin for high-copy replication and maintenance of the plasmid in E. coli
  • Kanamycin resistance gene for selection in E. coli
  • The ccdB⁄chloramphenicol fusion gene located between the two attL sites for
    o negative selection and
    o Chloramphenicol selection in E. coli
  • Kanamycin resistance gene for selection in E. coli
    TRUSTED_SUSTAINABILITY
    • Specifications

    Specifications

    Dual SelectionExpression Vector
    Chloramphenicol (CmR), Kanamycin (KanR)
    Untagged
    10 μg
    pENTR™
    10 μg pENTR™ Dual Selection vector, in 20 ul in TE buffer, pH 8.0.
    Store at -20°C
    No Cleavage Site
    Gateway
    pENTR
    Product Suggestions

    Product Suggestions

    Videos
    SDS
    Documents

    Documents

    Certificates
    Special Offers

    Special Offers

    For Research Use Only. Not for use in diagnostic procedures.