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Gibco™ Medium 199, Earle's Salts
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Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Brand:  Gibco™ 31150030

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Product Code. 11554426

  • 2720.00 SEK / 5000mL

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Medium 199 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

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Specifications

Specifications

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Rat epithelial
1X
Medium 199
Glutamine, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate
Sustainable packaging
290 - 310 mOsm/kg
10 x 500 mL
CO2
Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues
Liquid
Sterile-filtered
No HEPES
cGMP-compliant under the ISO 13485 standard
Gibco™
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For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.