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Invitrogen™ Plasmid pBR322

Prepacked column

Brand:  Invitrogen™ 15367014

1596.00 SEK valid until 2024-12-31
Use promo code "21651" to get your promotional price.

Product Code. 11598726

  • 2100.00 SEK / 25µg

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For Research Use Only. All usage must comply with product instructions.


Includes: pBR322 is supplied at 0.25μg/μL in 10mM Tris-HCl (pH 7.4), 5mM NaCl, 0.1mM EDTA.
This item is not returnable. View return policy

Description

Description

Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !-lactamase (AmpR) gene and eight unique sites within the TetR gene. The plasmid, purified from DH10B E. coli, also has 14 non-selectable unique restriction endonuclease recognition sites. Unique cleavage sites for Hind III and Cla I are found in the promoter of the tetracycline resistance gene. Insertion of DNA at either of these two sites usually results in loss of tetracycline resistance. Performance and Quality Testing: Purity, DNA structure, and selected restriction endonuclease fragmentation patterns are verified by agarose gel analysis. Transformation of E. coli HB101 to confirm AmpR and TetR is confirmed by plating on selective medium.

Cloning, Restriction Enzyme Cloning

Order Info

Approved for shipment on Wet or Dry ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Restriction Enzyme/ MCS
pBR322 is supplied at 0.25 μg/μl in 10 mM Tris-HCl (pH 7.4), 5 mM NaCl, 0.1 mM EDTA. Store at +4°C.
Plasmid
Approved for shipment on Wet or Dry Ice
0.25 μg/μl
Cloning
25 μg
pBR322
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For Research Use Only. Not for use in diagnostic procedures.