missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Extraction
  5. Thermo Scientific™ Y-PER™ Yeast Protein Extraction Reagent
  6. 11824121

Thermo Scientific™ Y-PER™ Yeast Protein Extraction Reagent

Release functionally active solubilized proteins with the first commercially available yeast lysis reagent to use a mild detergent lysis procedure.

Brand:  Thermo Scientific™ 78991

1400.12 SEK valid until 2024-12-31
Use promo code "21651" to get your promotional price.


 View more versions of this product

Product Code. 11824121

  • 1972.00 SEK / 200mL
In Stock
to see stock.
Explore more special offers
Add to basket
This item is not returnable. View return policy

Description

Description

Thermo Scientific™ Y-PER Yeast Protein Extraction Reagent is the first commercially available yeast lysis reagent to use a mild detergent lysis procedure to efficiently release functionally active solubilized proteins.

This detergent-based cell lysis buffer eliminates the need to use glass beads or mechanical disruption to break through the thick proteinaceous cell envelope to extract protein Y-PER Reagent is effective for S. cerevisiae and other popular species, making it applicable for use in fusion-tagged protein purification and reporter enzyme assays with these model organisms. The lysis reagent also can be used for genomic and plasmid DNA extraction from yeast. The standard protocol for protein extraction is easy: simply add an appropriate volume of Y-PER Reagent to pelleted yeast cells, incubate at room temperature for approximately 20 minutes and spin down the debris. The resulting supernatant is a concentrated protein solution, surpassing typical yields obtained with traditional glass bead disruption.

Highlights:

  • More protein – extracts more than twice as much protein as glass bead methods

  • Less hassle – eliminates the difficulties associated with traditional glass bead lysis (e.g., clinging static-charged beads, protein/bead clumps and runaway beads)

  • Optmized for yeast – validated for use with Saccharomyces cerevisiae, Schizosaccharomyces pombe and Pichia pastoris

  • Versatile – effective for many different organisms, including Bacillus subtilus and both Gram-positive and Gram-negative bacteria; suitable for use in a diverse range of situations

TRUSTED_SUSTAINABILITY
Specifications

Specifications

200 mL
Yeast Protein Extraction Reagent
Store product at room temperature.
Liquid
200 mL
Y-PER™
Product Suggestions

Product Suggestions

Videos
Safety and Handling

Safety and Handling

Product Identifier
  • Y-PER Yeast Protein Extraction Reagent

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2

Hazard Statement
  • H319-Causes serious eye irritation.

Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental information
  • MIXTURE LIST-Contains : glycerol, disodium hydrogenorthophosphate

SDS
Documents

Documents

Certificates
Special Offers

Special Offers

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.