Life Science Buffers
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Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
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Gibco™ DPBS, no calcium, no magnesium
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DPBS, no calcium, no magnesium
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Dilution | 1 X |
---|---|
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | DPBS |
Gibco™ PBS, pH 7.2
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PBS, pH 7.2
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Dilution | 1 X |
---|---|
Content And Storage | 15°to 30°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.2 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 280 to 320 mOsm/kg |
Gibco™ PBS (10X), pH 7.4
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PBS (10X), pH 7.4
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 10 X |
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
Capacity | 500 mL |
pH | 7.3 to 7.5 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2800 to 3100 mOsm/kg |
Gibco™ PBS, pH 7.4
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Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Ambient |
---|---|
Dilution | 1 X |
Content And Storage | 15°C to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.3 to 7.5 |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 280 - 315 mOsm/kg |
Tris Buffered Saline Tablets, pH 7.6, Medicago
Supplied as exactly pre-weighed tablets. Medicago Tris Buffered Saline Tablets, pH 7.6 gives 500ml of 1x solution with 0.05M Tris-HCl buffer and 0.15M sodium chloride. Manufactured in ISO 9001 and 13485 certified facilities.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!
Gibco™ DPBS (10X), no calcium, no magnesium
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 36 months from date of manufacture |
Form | Liquid |
Color | Clear |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 2630 - 3000 mOsm/kg |
Dilution | 10 X |
Additive | No Sodium Pyruvate |
Product Type | DPBS |
Classification | Animal Origin-Free |
pH | 6.7 - 7.0 |
Solution Type | Dulbecco's Phosphate Buffered Saline |
Product Line | Gibco™ |
Gibco™ PBS (10X), pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 24 months from date of manufacture |
Form | Liquid |
Color | White |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 2800 - 3100 mOsm/kg |
Dilution | 10 X |
Additive | No Sodium Pyruvate |
Product Type | Phosphate Buffered Saline (PBS) |
Classification | Animal Origin-Free |
pH | 7.4 |
Solution Type | Phosphate Buffered Saline |
Product Line | Gibco™ |
Thermo Scientific™ Pierce™ Concentrated Buffer Stocks (10X and 20X)
Save time using Pierce Concentrated Buffer stock solutions (10X and 20X) with simple dilutions to expedite your experiment without sacrificing quality or consistency.
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Gibco™ HBSS (10X), calcium, magnesium, no phenol red
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Tris Hydrochloride, 1M Solution (pH 7.5/Mol. Biol.), Fisher BioReagents™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
SMILES | C(C(CO)(CO)N)O |
Synonym | Tris-HCl |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
Invitrogen™ PBS (Phosphate-Buffered Saline) Tablets
Phosphate-buffered saline (PBS) is a balanced salt formulation used for a variety of cellular applications.
Gibco™ HEPES (1M)
Greener Choice Product
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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HEPES (1M)
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Gibco™ DPBS (10X), no calcium, no magnesium
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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DPBS (10X), no calcium, no magnesium
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Dilution | 10 X |
---|---|
Content And Storage | 15°to 30°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
pH | 6 to 7 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2500 to 2900 mOsm/kg |