Gel Electrophoresis Buffers and Diluents
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Gel Electrophoresis Buffers and Diluents
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Thermo Scientific™ DNA Gel Loading Dye (6X)
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Prepare DNA markers and samples for loading on agarose or polyacrylamide gels with Thermo Scientific™ 6X DNA Loading Dye.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Invitrogen™ NuPAGE™ LDS Sample Buffer (4X)
Used to prepare protein samples for denaturing gel electrophoresis with NuPAGE™ Novex™ gels. Invitrogen™ Novex™ NuPAGE™ LDS Sample Buffer (4X) contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Approved for shipment at Room Temperature or on Wet Ice |
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Buffer | Sample Loading Buffers |
Content And Storage | Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red. Store at 4°C to 25°C. |
Gel Type | NuPAGE |
Product Type | LDS Sample Buffer |
Gel Compatibility | NuPAGE™ Gels |
Product Line | NuPAGE™ |
Invitrogen™ NuPAGE™ Antioxidant
Proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis
Invitrogen™ NuPAGE™ Sample Reducing Agent (10X)
Ready-to-use reagent to reduce protein samples for protein gel electrophoresis
Shipping Condition | Wet Ice |
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Content And Storage | NuPAGE™ Sample Reducing Agent (500 mM dithiothreitol) should be stored at 4°C. Guaranteed stable for 6 months unless otherwise specified in the product literature. |
Product Type | Sample Reducing Agent |
Shelf Life | 6 Months |
Concentration | 500 mM |
Product Line | NuPAGE™ |
Invitrogen™ NuPAGE™ Tris-Acetate SDS Running Buffer (20X)
Formulated for separation of proteins, in their denatured state, on NuPAGE Novex Tris-Acetate gels
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Invitrogen™ NuPAGE™ Transfer Buffer (20X)
Use to transfer proteins from NuPAGE Novex gels to membranes for Western blotting
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Room Temperature |
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Buffer | Transfer Buffers |
Content And Storage | Store at room temperature. Guaranteed stable for 6 months unless otherwise specified in the product literature. |
Gel Type | NuPAGE |
Product Type | Transfer Buffer |
Shelf Life | 6 Months |
Gel Compatibility | NuPAGE™ Gels |
Product Line | NuPAGE™ |
Invitrogen™ 4X Bolt™ LDS Sample Buffer
Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Buffer | Sample Loading Buffers |
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Content And Storage | Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red. Store at 4°C to 25°C. |
Gel Type | Bis-Tris Plus |
Product Type | LDS Sample Buffer |
For Use With (Equipment) | Bolt Mini Gel Tank |
Concentration | 4X |
Gel Compatibility | Bolt™ Bis-Tris Plus Gels |
Product Line | Bolt™ |
Macherey-Nagel™ NucleoBond™ PC EF Kit Buffer Solutions
Additional supply of buffer for use with NucleoBond™ PC EF kits (PC 500 EF, PC 2000 EF, PC 10000 EF, PC Prep 100) for the purification of endotoxin-free plasmid DNA. Buffer solutions include: S1/S2/S3-EF, N2/N3/N4/N5-EF, TE-EF, and H2O-EF.
Content And Storage | Room Temperature (18°C to 25°C) |
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CAS | 64-17-5d |
Form | Liquid |
For Use With (Equipment) | NucleoBond™ PC EF kits (PC 500 EF, PC 2000 EF, PC 10000 EF, PC Prep 100) |
Color | Colorless |
Shelf Life | At least 1 year |
For Use With (Application) | Purification of endotoxin-free plasmid DNA |
Invitrogen™ 10X Bolt™ Sample Reducing Agent
Optimized for use with Bolt™ Bis-Tris Plus gels, and available in a variety of formats.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Content And Storage | Sample Reducing Agent (10X) contains 500 mM dithiothreitol (DTT). Store at 2°C to 8°C. |
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Form | Liquid |
Product Type | Sample Reducing Agent |
For Use With (Equipment) | Bolt Mini Gel Tank |
Formulation | 500 mM dithiothreitol (DTT) |
Reagent Type | Reducing Agent |
Concentration | 10X, 500 mM |
Gel Compatibility | Bolt™ Bis-Tris Plus Gels |
Product Line | Bolt™ |
Invitrogen™ UltraPure™ SSC, 20X
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
For use in nucleic acid hybridizations and blot transfer applications
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Shipping Condition | Room Temperature |
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Content And Storage | Store at room temperature. |
Form | Liquid |
Product Type | SSC |
Concentration | 20 X |
For Use With (Application) | Nucleic Acid Gel Electrophoresis, Blotting |
Product Line | UltraPure™ |
Invitrogen™ IEF Cathode Buffer pH 3-10 (10X)
An optimized pre-mixed IEF cathod buffer
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Invitrogen™ Novex™ IEF Sample Buffer pH 3-10 (2X)
An optimized pre-mixed IEF sample buffer
Invitrogen™ Novex™ IEF Anode Buffer (50X)
An optimized pre-mixed IEF anode buffer
Invitrogen™ Digitonin (5%)
Digitonin (5%), or digitin, is a non-ionic detergent with a cholesterol-like structure that can water-solubilize lipids, permeabilize cells, be used as a cytotoxicity positive control and solubilize membrane-bound proteins for NativePAGE™ protein analysis or cryo-EM structural analysis.
![EDGE](/content/dam/fishersci/glyphs/edge.png)
Invitrogen™ NativePAGE™ Sample Buffer (4X)
A solution used to solubilize native protein samples prior to use with the NativePAGE Gel System
![EDGE](/content/dam/fishersci/glyphs/edge.png)