All Primary Antibodies
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All Primary Antibodies
Primary antibodies, immunoglobulins that bind to specific proteins or other biomolecules, are used in many research applications and protocols to detect targets of interest. They are developed using different animal hosts, including mouse, rat, rabbit, goat, sheep, and many others.
Monoclonal and Polyclonal Antibodies
One type of primary antibodies, monoclonal antibodies, provides high reproducibility and low cross-reactivity and background noise. Another type, polyclonal antibodies, often costs less and provides greater affinity and quicker binding. Both are produced using plasma B cells, but the former uses the same clone and the latter uses different clones. Monoclonal antibodies require hybridoma cell lines, and polyclonal antibodies do not.
There are also recombinant monoclonal antibodies with similar benefits, like high affinity, scalability, and specificity. They are produced using in vitro cloning of plasma B cells and expression hosts.
Conjugated Primary Antibodies
Antibodies can be labeled with various fluorophores or detection agents or used without labels. Labeled primary antibodies, also known as conjugated primary antibodies, help researchers simplify and streamline their applications. They are coupled with common enzymes and dyes such as Alexa Fluor and often used in protein and cell analysis.
Applications
Antibodies for life sciences applications are used in flow cytometry, western blotting, ELISA, immunohistochemistry, and immunocytochemistry. Secondary antibodies can be added to support the detection and purification of certain antigens. They bind to the primary antibody, which binds to the antigen of interest. Finding the right combination of antibodies can result in greater antigen specificity and a strong, detectable signal.
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Filtered Search Results
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Antigen | Fc Block |
---|---|
Regulatory Status | RUO |
Content And Storage | Store undiluted at 4°C |
Target Species | Human,Rhesus Monkey |
Conjugate | Unconjugated |
Applications | Flow Cytometry |
Form | Purified |
Classification | Monoclonal |
Primary or Secondary | Primary |
Concentration | 0.5mg/mL |
Clone | 3070 |
Streptavidin APC, BD
Research applications include studies of staining by indirect immunofluorescence when used with biotinylated antibodies.
Content And Storage | Store undiluted at -20°C. |
---|---|
Description | Survival Motor Neuron |
Target Species | Canine,Human,Mouse,Rat |
Host Species | Mouse |
Conjugate | Unconjugated |
Applications | Western Blot |
Isotype | IgG1 |
Research Discipline | Cell Biology |
Concentration | 250μg/mL |
Antigen | SMN |
Regulatory Status | RUO |
Purification Method | Affinity Purified |
Formulation | Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide. |
Immunogen | Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide. |
Classification | Monoclonal |
Primary or Secondary | Primary |
Clone | 8 |
Content And Storage | Store undiluted at -20°C. |
---|---|
Target Species | Canine,Chicken,Human,Mouse,Rat |
Host Species | Mouse |
Conjugate | Unconjugated |
Applications | Western Blot |
Isotype | IgG1 |
Research Discipline | Cell Biology |
Concentration | 1.0mg/mL |
Antigen | Paxillin |
Regulatory Status | RUO |
Purification Method | Affinity Purified |
Formulation | Aqueous buffered solution containing glycerol and ≤0.09% sodium azide. |
Immunogen | Chicken Paxillin aa. 1 to 557 |
Classification | Monoclonal |
Primary or Secondary | Primary |
Clone | 349 |
Antigen | CD45 |
---|---|
Regulatory Status | RUO |
Purification Method | Affinity Purified |
Target Species | Human |
Host Species | Mouse |
Conjugate | Brilliant Ultraviolet 395 |
Immunogen | Human Peripheral Blood Leucocytes |
Classification | Monoclonal |
Primary or Secondary | Primary |
Clone | HI30 |
Antigen | CD1a |
---|---|
Regulatory Status | RUO |
Purification Method | Affinity Purified |
Target Species | Human |
Host Species | Mouse |
Conjugate | Brilliant Violet 421 |
Classification | Monoclonal |
Primary or Secondary | Primary |
Clone | HI149 |
Antigen | CD16 |
---|---|
Regulatory Status | RUO |
Purification Method | Affinity Purified |
Target Species | Baboon,Cynomolgus,Human,Rhesus Monkey |
Host Species | Mouse |
Conjugate | Brilliant Ultraviolet 395 |
Immunogen | Human polymorphonuclear leukocytes |
Classification | Monoclonal |
Primary or Secondary | Primary |
Clone | 3G8 |