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Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent
Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.
Cytiva Whatman™ Polycap Disposable Capsules, 36 AS
Provides highly effective filtration area in a small size. Whatman™ Polycap Disposable AS Capsules, AS 36 filters large volumes and difficult samples with ease.
Housing Material | Polypropylene (PP) |
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Material (Membrane) | Nylon |
For Use With (Application) | Filtration of aqueous solutions (such as cell culture media, serum enzymes and water) and removal of bacteria and debris. |
Max. Pressure | 4.1 bar (60 psi) |
Filtration Area | 400 cm2 |
Cytiva HisTrap™ FF Columns: 16 x 25mm
Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.
Flow Rate | <20 mL/min. |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C, 20% Ethanol |
Diameter (English) Inner | 0.6 in. |
Description | HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Diameter (Metric) Inner | 16 mm |
Material | Polypropylene |
Support Type | 6% Cross-linked Agarose |
Cytiva HyClone™ Dulbecco's Phosphate Buffered Saline, Solution
Buffer cell culture with PBS in solution.
Regulatory Status | RUO |
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Content And Storage | 15° to 30°C |
Sterilization Method | 0.1 μm Sterile-filtered |
Product Type | Dulbecco's Phosphate Buffered Saline |
Form | Liquid |
Endotoxin Level | ≤0.025EU/mL |
pH | 7 to 7.6 |
Shelf Life | 24 Months |
Certifications/Compliance | Manufactured under cGMP and ISO 9001 certified facilities |
For Use With (Application) | Serves as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of time |
Osmolality | 276±10mOsm/kg H2O |
Sterility | Sterile |
Cytiva Amersham™ ECL™ Western Blotting Detection Reagents
Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used.
Content And Storage | 2°C to 8°C |
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Sensitivity | ∼10 pg |
Product Type | ECL Western Blot Detection Reagent |
For Use With (Application) | Western Blot |
Cytiva Whatman™ Polycap HD Disposable Capsules, 150 HD
Offers high filtration efficiency and excellent filtrate purity
Quantity | 5/Pk. |
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For Use With (Application) | Polycap HD 150 |
Cytiva Whatman™ Polycap™ HD Disposable Capsules: 75 HD
Offers high filtration efficiency and excellent filtrate purity due to its materials and methods of manufacture. Whatman™ Polycap™ HD Disposable Capsules fit between gross filters and microporous membrane filters used for final filtration.
Housing Material | Polypropylene (PP) |
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Material (Membrane) | Monofilament Anisotropic Polypropylene |
For Use With (Application) | Filtration of high-debris solutions and prefiltration of aggressive chemical solutions. Offer broad capacity between gross paper filters and microporous membranes. |
Autoclavable | Autoclavable |
Filtration Area | 805 cm2 |
Cytiva Whatman™ Polycap Disposable Capsules, 75 AS
Polycap 75 AS filters for filtration of aqueous solutions
Material (Membrane) | Nylon |
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For Use With (Application) | Filtration of aqueous solutions (such as cell culture media, serum enzymes and water) and removal of bacteria and debris. |
Cytiva Whatman™ Nuclepore™ Hydrophilic Membrane
A highly defined pore size cutoff in the sub-micron range enables particle deformity measurements, and the capture of extremely small particles
Type | Track Etched Hydrophilic Membrane Filters |
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Description | Circles |
Hydrophobicity | Hydrophilic |
For Use With (Application) | Bioassays, Cell Biology and Environment Analysis |
Membrane Type | Polycarbonate Film Track-Etched (PFTE) |
anti-Rabbit IgG, peroxidase-linked species-specific whole antibody (from donkey) Secondary Antibody, Cytiva
A highly species-specific anti-Rabbit IgG. Cytiva Lifescience™ anti-Rabbit IgG, peroxidase-linked species-specific whole antibody (from donkey) Secondary Antibody is used with Amersham™ ECL Western Blotting detection reagents.
Antigen | IgG |
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Purification Method | Affinity Purified |
Host Species | Donkey |
Conjugate | HRP |
Classification | Monoclonal |
Isotype | IgG |
Primary or Secondary | Secondary |
anti-Mouse IgG, peroxidase-linked species-specific whole antibody (from sheep) Secondary Antibody, Cytiva
Highly species-specific anti-Mouse IgG. Cytiva Lifescience™ anti-Mouse IgG, peroxidase-linked species-specific whole antibody (from sheep) Secondary Antibody is designed for applications such as protein blotting, ELISA and immunocytochemistry.
Antigen | IgG, Whole Molecule |
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Purification Method | Affinity Purified |
Host Species | Sheep |
Conjugate | HRP |
Classification | Monoclonal |
Isotype | IgG |
Primary or Secondary | Secondary |
Cytiva ExcelGel 12.5%, Tris-Acetate, 0.5 mm, Protein Gel, 25-well
Separation Range: 15 to 40 kd; Separation Type: Denaturing; For Use With: Immobiline DryStrip Gels